Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-alpha release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. Moreover, the peptide fractions were able to significantly increase the wound repair in IEC-6. The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level.

Anti-inflammatory and antioxidant properties of dehydrated potato-derived bioactive compounds in intestinal cells / Basilicata, Manuela Giovanna; Pepe, Giacomo; Rapa, Shara Francesca; Merciai, Fabrizio; Ostacolo, Carmine; Manfra, Michele; Di Sarno, Veronica; Autore, Giuseppina; De Vita, Daniela; Marzocco, Stefania; Campiglia, Pietro. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 20:23(2019), pp. 1-15. [10.3390/ijms20236087]

Anti-inflammatory and antioxidant properties of dehydrated potato-derived bioactive compounds in intestinal cells

De Vita, Daniela;
2019

Abstract

Inflammation and oxidative stress are always more recognized as responsible for chronic disease at the intestinal level. Currently, a growing interest is addressed to the discovery of diet-derived products which have anti-inflammatory and antioxidant properties. This work aims to characterize the pharmacological potential of dehydrated potatoes. For this purpose, a simulated gastrointestinal digestion was carried out. The bioaccessible peptides were fractionated on the basis of their molecular weight and tested on intestinal epithelial cells (IEC-6) under oxidative and inflammatory conditions. Our results demonstrate that the tested peptide fractions were able to significantly inhibit tumor necrosis factor-alpha release and cycloxygenase-2 and inducible nitric oxide synthase expression. The tested peptides also showed significant antioxidant activity, being able to both reduce reactive oxygen species (ROS) release, also from mitochondria, and nitrotyrosine formation, and increase the antioxidant response by heme oxygenase-1 and superoxide dismutase expression. Moreover, the peptide fractions were able to significantly increase the wound repair in IEC-6. The obtained results indicate the anti-inflammatory and antioxidant potential of dehydrated potatoes at the intestinal level.
2019
bioactive peptides; in vitro gastrointestinal digestion; inflammation; intestinal epithelial cells; oxidative stress; potato proteins; animals; anti-inflammatory agents; antioxidants; cell line; cell movement; cell survival; cyclooxygenase 2; Digestion; Gastrointestinal Tract; Heme Oxygenase-1; Interferons; Intestines; Lipopolysaccharides; Nitric Oxide Synthase Type II; Peptides; Phytochemicals; Rats; Reactive Oxygen Species; Solanum tuberosum; Stress, Mechanical; Superoxide Dismutase; Tumor Necrosis Factor-alpha; Tyrosine; desiccation
01 Pubblicazione su rivista::01a Articolo in rivista
Anti-inflammatory and antioxidant properties of dehydrated potato-derived bioactive compounds in intestinal cells / Basilicata, Manuela Giovanna; Pepe, Giacomo; Rapa, Shara Francesca; Merciai, Fabrizio; Ostacolo, Carmine; Manfra, Michele; Di Sarno, Veronica; Autore, Giuseppina; De Vita, Daniela; Marzocco, Stefania; Campiglia, Pietro. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 20:23(2019), pp. 1-15. [10.3390/ijms20236087]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1404923
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